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Genetic association between the polymorphism of cytosolic PLA2 gene family and schizophrenia

Qiong YU PhD, Xiang-Fei MENG PhD, Jie-Ping SHI, Ya-Qin YU PhD,

《医学前沿（英文）》 2010年第4卷第1期页码 101-105 doi: 10.1007/s11684-010-0017-6

摘要： Abnormal phospholipid metabolism in the brain plays an important role in neuropsychiatric diseases. Phospholipase A2 (PLA2) is a crucial element for normal neuro-physiological function. This study aims to investigate the genetic association between the polymorphism of cytosolic phospholipase A2 (cPLA2) family genes and schizophrenia among Han Chinese in the northern part of China. The polymerase chain reaction-based ligase detection reaction (PCR-LDR) was applied to detect the genotype ten single nucleotide polymorphisms (SNPs) of cPLA2 family genes among 201 pedigrees consisting of fathers, mothers and affected offsprings with schizophrenia. The pedigrees were collected from 2000 to 2006. Haplotype relative risk (HRR) test, transmission disequilibrium test (TDT), haplotype transmission analysis and multiple locus analysis were conducted to analyze the genotyping data. The genotypic frequency of cPLA2 gene did not deviate from Hardy-Weinberg equilibrium either in case or control group. HRR and TDT showed that the ten SNPs were not associated with schizophrenia (>0.05). Analysis for haplotype transmission showed that no haplotype system was associated with schizophrenia (>0.05). The conditioning on allele (COA) and conditioning on gene (COG) tests showed disease associations with the haplotype of rs2162886-rs1668589, rs891014-rs1668589 and rs2307279-rs7542180 (χ=6.913, =0.032; χ=8.393, =0.015; χ=8.447, =0.038). Our data suggest that many loci in the cPLA2 family genes may be associated with schizophrenia.

关键词： schizophrenia cytosolic phospholipase A2 ligase detection reaction polymorphism

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MB（A2/O）工艺的好氧、缺氧摄磷及微生物特性

吴剑,王世和

《中国工程科学》 2007年第9卷第11期页码 191-195

摘要：

为开发高效除磷脱氮技术，研制了将MBR与A²/O工艺有机集成的新型MB（A²/O）组合工艺。研究了MB（A²/O）工艺处理城市污水的好氧、缺氧摄磷性能及微生物特性，并分析了其机理。结果表明：在 MB（A²/O）系统中，聚磷菌约占活性污泥总量的20%~40%，其中大量存在能够利用NOX－N作为电子受体进行反硝化除磷的DPB，约占聚磷菌数量的35. 66%~67好氧摄磷的平均速率为2. 30mgP·gMLSS^-1·h^-1，最大摄磷速率为5. 44mgP·gMLSS^-

关键词：好氧摄磷反硝化除磷聚磷菌电子受体

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转录因子HNF1A、HNF4A和FOXA2调节肝细胞蛋白质N-糖基化 Article

Vedrana Vičić Bočkor,Nika Foglar,Goran Josipović,Marija Klasić,Ana Vujić,Branimir Plavša,Toma Keser,Samira Smajlović,Aleksandar Vojta,Vlatka Zoldoš

《工程（英文）》 2024年第32卷第1期页码 58-69 doi: 10.1016/j.eng.2023.09.019

摘要：

Hepatocyte nuclear factor 1 alpha (HNF1A), hepatocyte nuclear factor 4 alpha (HNF4A), and forkhead box protein A2 (FOXA2) are key transcription factors that regulate a complex gene network in the liver, creating a regulatory transcriptional loop. The Encode and ChIP-Atlas databases identify the recognition sites of these transcription factors in many glycosyltransferase genes. Our in silico analysis of HNF1A, HNF4A, and FOXA2 binding to the 10 candidate glyco-genes studied in this work confirms a significant enrichment of these transcription factors specifically in the liver. Our previous studies identified HNF1A as a master regulator of fucosylation, glycan branching, and galactosylation of plasma glycoproteins. Here, we aimed to functionally validate the role of the three transcription factors on downstream glyco-gene transcriptional expression and the possible effect on glycan phenotype. We used the state-of-the-art clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) molecular tool for the downregulation of the HNF1A, HNF4A, and FOXA2 genes in HepG2 cells—a human liver cancer cell line. The results show that the downregulation of all three genes individually and in pairs affects the transcriptional activity of many glyco-genes, although downregulation of glyco-genes was not always followed by an unambiguous change in the corresponding glycan structures. The effect is better seen as an overall change in the total HepG2 N-glycome, primarily due to the extension of biantennary glycans. We propose an alternative way to evaluate the N-glycome composition via estimating the overall complexity of the glycome by quantifying the number of monomers in each glycan structure. We also propose a model showing feedback loops with the mutual activation of HNF1A–FOXA2 and HNF4A–FOXA2 affecting glyco-genes and protein glycosylation in HepG2 cells.

关键词： Clustered regularly interspaced short palindromic repeats/dead Cas9 (CRISPR/dCas9) Epigenetics Hepatocyte nuclear factor 1 alpha (HNF1A) Hepatocyte nuclear factor 4 alpha (HNF4A) Forkhead box protein A2 (FOXA2) N-glycosylation HepG2 cells

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第三届环境、工业和能源工程国际会议(EI2E 2019)

会议日期： 2019年09月19日

会议地点：宁夏银川

主办单位： APISE

第四届设计、机械和材料工程国际会议(D2ME 2019)

会议日期： 2019年09月26日

会议地点：釜山，韩国

主办单位： HKSME

肖永红：临床高耐药与高毒性病原体共进化（2023年2月22日）

2023年03月10日

关键词：代谢与免疫

第四届设计、机械和材料工程国际会议(D2ME 2019)

会议日期： 2019年09月26日

会议地点：釜山，韩国

主办单位： HKSME

赵家军：2型糖尿病患者的身体活动、久坐行为与心血管疾病风险的关系：MlDiab研究（2023年2月21日）

2023年03月02日

关键词：营养健康

沈世钊：大跨结构（2018年6月2日）

2021年04月22日

关键词：光学技术

Jian Li：iScience and Interdisciplinary Research（2020年4月2日）

2022年04月18日

关键词：工程管理

金涌：化学反应规模的扩大（2018年6月2日）

2021年04月22日

关键词：化工

徐銤院士解读中国核能（2018年6月2日）

2021年04月22日

关键词：核能

孙金生：中国油气开采（2018年6月2日）

2021年04月15日

关键词：采油工程

第2届智能医疗与图像处理国际会议(IMIP 2020)

会议日期： 2020年04月23日

会议地点：天津

主办单位： IMIP 2020

Improvement in the synthesis of 2-(5-amino-1,2,4-thiadiazol-3-yl)-2-(Z)-methoxyiminoacetic acid 2-benzothiazolyl

GAO Shihao, SUN Chenghui, ZHAO Xinqi, GAO Changquan

《化学科学与工程前沿（英文）》 2008年第2卷第1期页码 80-84 doi: 10.1007/s11705-008-0017-0

摘要： 2-(5-Amino-1,2,4-thiadiazol-3-yl)-2-(Z)-methoxyiminoacetic acid 2-benzothiazolyl thioester(III), an important intermediate of the fourth generation cephalosporins, was efficiently synthesized by reacting 2-(5-amino-1,2,4-thiadiazol-3-yl)-2-(Z)-methoxyiminoacetic acid (I) with 2,2′-dibenzothiazole disulfide (II) in the presence of triphenylphosphine. Effects of reaction time, temperature, solvents, catalysts and feeding molar ratio on the yield and quality of products were investigated, and an improved procedure suitable for industrial production was established. Using 1,2-dichloroethane as solvent, triphenylphosphine as reducer, and triethylamine as catalyst, (I) : (II) : (triphenylphosphine) = 1.0 : 1.0 : 1.0, the product was obtained at room temperature in 98.1% yield. The purity of the product without further purification is 98.7% determined by HPLC method. This procedure could be a suitable alternative to the traditional processes because of its easy handling, high yield and low cost.

关键词： 5-Amino-1 2 4-thiadiazol-3-yl 1 2-dichloroethane important intermediate 2-benzothiazolyl thioester temperature